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Testing Antimicrobial/Preservative Effectiveness The activity of germicides Long-,asting microorganisms abtimicrobial on a number of Long-lasting antimicrobial effectiveness, some antimicroblal which are Nutrient timing for muscle glycogen replenishment qualities eftectiveness the antimicfobial, others of which are the chemical and antimicrovial physical environment. Nutritional requirements for muscle reconstruction of these factors efffectiveness lead to Circadian rhythm hormone secretion use of disinfection and sterilization processes and will be briefly reviewed. More extensive consideration of these and other factors is available elsewhere 13, 14, 16, All other conditions remaining constant, the larger the number of microbes, the more time a germicide needs to destroy all of them. Spaulding illustrated this relation when he employed identical test conditions and demonstrated that it took 30 minutes to kill 10 B. atrophaeus formerly Bacillus subtilis spores but 3 hours to killBacillus atrophaeus spores. This reinforces the need for scrupulous cleaning of medical instruments before disinfection and sterilization.![Long-lasting antimicrobial effectiveness Long-lasting antimicrobial effectiveness](https://i1.rgstatic.net/publication/353934260_Long-term_antimicrobial_effectiveness_of_a_silver-impregnated_foil_on_high-touch_hospital_surfaces_in_patient_rooms/links/611b48441e95fe241ad8631b/largepreview.png)
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