How we reviewed Prebjotic article: Preboitic. SCFAs are Propergies aliphatic organic acids that have from one to six Habits for athletic performance atoms, such as acetate C2propionate C3and butyrate C4and are the final products of bacterial fermentation processes. Supplier Information. Oliveira DL, Costabile A, Wilbey RA, Grandison AS, Duarte LC, Roseiro LB. Sreenivas KM, Lele SS.

Prebiotic properties -

Other studies evaluating the prebiotic potential of food using in vitro models are described in Table 1. It has been well established that the colon microbiota has a deep influence on health.

The study of the prebiotic potential in humans would be considered as a gold standard in case of absence of ethical and practical limitations, which may make the research unreliable or limited, in addition to the high dropout rates of study participants. Thus, animal models become an alternative to study the prebiotic potential of foods, since they allow direct access to intestinal contents as well as to organs and tissues [ 61 ].

Usually, the animal models used for the study of gut microbiota are swine [ 62 ], zebrafish [ 63 ], and more widely in rodents such as rats [ 47 ], hamsters [ 64 ], and mice [ 53 ], especially when the potential prebiotic of foods is evaluated. Samal et al. added at different concentrations to the diet of rats for 12 weeks and observed that the consumption promoted beneficial effects on immunity, intestinal morphometry, and hindgut fermentation of rats.

Supplementation with 2. and Bifidobacterium spp. in hamsters [ 64 ]. Not only should the gut microbiota be evaluated in in vivo studies but also other variables such as pH, feces humidity, and SCFAs production, which is directly related to the selective bacterial fermentation of prebiotics [ 66 , 67 ].

These microbial metabolites can be used as sources of energy by the host and can also act as regulators of energy consumption and metabolism [ 69 ]. pH acidification can also be an indicator of fermentation of prebiotic components of foods in the colon by endogenous bacteria and production of organic acids directly responsible for this process [ 70 , 71 ].

In addition, the preservation of the intestinal epithelium in healthy rats or its recovery in diseased rats may provide evidence of the prebiotic potential, as observed by Hu et al.

Bränning et al. The consumption of diet containing blueberry husk by rats for 5 days resulted in higher amounts of propionic acid and butyric acid in the distal colon and feces, respectively, when compared to rats that were fed a control diet without fibers. Both acids are essential substrates for colonic epithelial cells, improving gut health, and a surplus of substrates which also have metabolic effects.

However, blueberry husk has antimicrobial effects, as observed by the decreased counts of lactobacilli, bifidobacteria, and enterobacteriaceae, and the larger pool of succinic acid may be a consequence of these antimicrobial effects.

In this model, blueberry husks do not demonstrate prebiotic properties. In healthy rats, the administration of the combination of FOS and RS induced changes in the intestinal microbiota and increased lactobacilli and bifidobacteria in caecum and colonic contents. In addition, treatment increased the moisture content and decreased the pH of caecum and colon.

The beneficial effects observed with this combination were confirmed in the healthy or colitis rats. Comparing CONT and CELL, caecal concentrations of fermentation products increased from 1.

This research concluded that the presence of soluble fermentable fiber appears to be more important than its source [ 75 ].

Other studies evaluating the prebiotic potential of foods using in vivo models are described in Table 2. The modulations of the intestinal microbiota and SCFA production are associated with many beneficial effects about the ingestion of prebiotics and isolated or added to foods, such as regulation of various physiological processes e.

Everard et al. Salazar et al. Thus, the prevalence of metabolic syndrome was lower in participants who ingested larger amounts of insoluble fibers [ 83 ]. Barbalho et al. Obese rats fed with hyperlipid diet and diet added of lyophilized jabuticaba peel rich in anthocyanins exhibited increased HDL and improved insulin resistance, suggesting that the diet added of this byproduct may have a protective effect against cardiovascular diseases by increasing HDL levels [ 86 ].

Changes in the intestinal microbiota may also influence the homeostasis of the immune [ 35 ], renal [ 88 ], and nervous systems [ 89 ], as well as the development and progression of pathophysiological processes such as hypertension [ 90 ] and colorectal cancer [ 91 ].

Healthy men and women daily supplied with FOS or GOS for 3 weeks showed decreased response to cortisol awakening, protecting against the risk of depression [ 93 ]. Rats with chronic kidney disease CKD fed for 3 weeks with RS diets had a delay in CKD progression and increased creatinine clearance when compared to CKD mice that received amylopectin [ 94 ].

The inclusion of prebiotics in industrialized foods has become a viable and healthy alternative, since there is a great demand of consumers for functional foods that can help in maintaining health.

In general, prebiotics are added to bakery products, breakfast cereals, beverages e. Prebiotics also have gelling properties e. Among prebiotics commonly used in the food industry, GOS are more stable at high temperatures and low pH mainly due to the beta bonds of their structure, which provide greater hydrolysis stability compared to FOS and inulin [ 96 ].

A type of RS known as RS3 can be added to fried battered products to increase the content of dietary fibers and avoid reducing moisture and the absorption of fats, since RS3 is very resistant to frying temperatures [ 97 ]. The importance of the consumption of prebiotics is unquestionable and they should be part of healthy diet.

In this sense, the development of foods added due to prebiotics by the industry can be advantageous due to the demand and profitability of this market, as well as for consumers who will have healthy foods available that can be readily consumed for the prevention or treatment of diseases, thus reducing public health costs.

However, there is no consensus on the recommended quantity of specific prebiotics for consumption in the diet, and this limitation is a major challenge regarding the different in vitro and in vivo models used to test the prebiotic potential of foods.

Both in vivo and in vitro models have helped advances of researches aimed at evaluating the prebiotic potential of foods through the composition and metabolism of the intestinal microbiota and their interactions. However, it is noteworthy that there are no ideal models, and the most adequate are those based on the study objectives and using association of complementary techniques.

Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution 3. Edited by María Chávarri Hueda. Open access peer-reviewed chapter Models to Evaluate the Prebiotic Potential of Foods Written By Jailane de Souza Aquino, Kamila Sabino Batista, Francisca Nayara Dantas Duarte Menezes, Priscilla Paulo Lins, Jessyca Alencar de Sousa Gomes and Laiane Alves da Silva.

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Impact of this chapter. Keywords functional foods prebiotics chronic diseases animal models intestinal microbiota.

Introduction Modern society has changed its standard of living every decade and today, health is becoming an increasingly important personal and social value. Food Main results References Oligosaccharides from Pitaya Hylocereus undatus Haw. Medjoul and apple bagasse Malus domestica var.

brevis, B. adolescentis, and the Weissella spp. Table 1. In vitro studies on the prebiotic potential of foods. Study model Foods Main results References Female rats Wistar Cocoa fibers Theobroma cacao L. Table 2. In vivo models for prebiotic food assessment.

References 1. Goetzke B, Nitzko S, Spiller A. Consumption of organic and functional food. Bachl T. Wellness trend benefits markets. Berlin: BVE; Chrysochou P. Food health branding: The role of marketing mix elements and public discourse in conveying a healthy brand image. Journal of Marketing Communications.

Food Quality and Standards Service, Food and Agriculture Organization. FAO Technical Meeting on Prebiotics. Food Quality and Standards Service AGNIS , Food and Agriculture Organization of the United Nations; 5. Sreenivas KM, Lele SS. Prebiotic activity of gourd family vegetable fibres using in vitro fermentation.

Food Bioscience. Gómez B, Gullón B, Yáñez R, Schols H, Alonso JL. Prebiotic potential of pectins and pectic oligosaccharides derived from lemon peel wastes and sugar beet pulp: A comparative evaluation. Journal of Functional Foods [Internet]. DOI: Scott KP, Martin JC, Duncan SH, Flint HJ.

FEMS Microbiology Ecology. Donelli G, Vuotto C, Mastromarino P. Phenotyping and genotyping are both essential to identify and classify a probiotic microorganism. Microbial Ecology in Health and Disease [Internet]. Guidelines for the Evaluation of Probiotics in Food.

London, Ontario, Canada: World Health Organization, Food and Agriculture Organization; Butel MJ. Probiotics, gut microbiota and health.

Médecine et Maladies Infectieuses [Internet]. He Z, Wang X, Li G, Zhao Y, Zhang J, Niu C, et al. Antioxidant activity of prebiotic ginseng polysaccharides combined with potential probiotic Lactobacillus plantarum C Rodrigues FC, Castro ASB, Rodrigues VC, Fernandes SA, Fontes EAF, de Oliveira TT, et al.

Yacon flour and Bifidobacterium longum modulate bone health in rats. Journal of Medicinal Food [Internet]. Estévez BG, Gullón P, Tavaria F, Yáñez R. Assessment of the prebiotic effect of quinoa and amaranth in the human intestinal ecosystem.

Maurer AD, Eller LK, Hallam MC, Taylor K, Reimer RA. Consumption of diets high in prebiotic fiber or protein during growth influences the response to a high fat and sucrose diet in adulthood in rats. Nutrition and Metabolism London. Scott KP, Gratz SW, Sheridan PO, Flint HJ, Duncan SH.

The influence of diet on the gut microbiota. Pharmacological Research [Internet]. Flint HJ, Duncan SH, Scott KP, Louis P. Links between diet, gut microbiota composition and gut metabolism. Proceedings of the Nutrition Society [Internet].

Gibson GR, Probert HM, Van Loo J, Rastall RA, Roberfroid MB. Dietary modulation of the human colonic microbiota: Updating the concept of prebiotics. Nutrition Research Reviews.

Marques TM, Cryan JF, Shanahan F, Fitzgerald GF, Ross RP, Dinan TG, et al. Innovative Food Science and Emerging Technologies.

Ufnal M, Pham K. Medical Hypotheses [Internet]. Principi N, Esposito S. Gut microbiota and central nervous system development. Journal of Infection [Internet]. Luna RA, Foster JA. Gut brain axis: Diet microbiota interactions and implications for modulation of anxiety and depression.

Current Opinion in Biotechnology [Internet]. Kabouridis PS, Pachnis V. Emerging roles of gut microbiota and the immune system in the development of the enteric nervous system.

Journal of Clinical Investigation. Sandrini S, Aldriwesh M, Alruways M, Freestone P. Journal of Endocrinology [Internet]. Candela M, Maccaferri S, Turroni S, Carnevali P, Brigidi P. Functional intestinal microbiome, new frontiers in prebiotic design.

International Journal of Food Microbiology [Internet]. American Dietetic Association. Position of the American Dietetic Association: Health implications of dietary fiber. Journal of the American Dietetic Association [Internet].

Cupersmid L, Fraga APR, de Abreu ES, Pereira IRO. Linhaça: Composição química e efeitos biológicos. Porfírio E, Henrique VSM, de Abreu Reis MJ. Brazilian Journal of Food Technology. Food Research International [Internet]. Kosmala M, Zduńczyk Z, Ju s ¨ kiewicz J, Jurgoński A, Karlińska E, Macierzyński J, et al.

Chemical composition of defatted strawberry and raspberry seeds and the effect of these dietary ingredients on polyphenol metabolites, intestinal function, and selected serum parameters in rats.

Journal of Agricultural and Food Chemistry. Roberfroid MB, Delzenne NM. Dietary fructans. Annual Review of Nutrition. Patel S, Goyal A. The current trends and future perspectives of prebiotics research: A review.

Ganapathy V, Thangaraju M, Prasad PD, Martin PM, Singh N. Current Opinion in Pharmacology. Parkar SG, Trower TM, Stevenson DE.

Fecal microbial metabolism of polyphenols and its effects on human gut microbiota. Anaerobe [Internet].

da Silva JK, Cazarin CBB, Colomeu TC, Batista ÂG, Meletti LMM, Paschoal JAR, et al. Antioxidant activity of aqueous extract of passion fruit Passiflora edulis leaves: In vitro and in vivo study.

Food Research International. The relationship between phenolic compounds from diet and microbiota: Impact on human health. Available from: www. Quiñones M, Miguel M, Aleixandre A. Beneficial effects of polyphenols on cardiovascular disease.

Pharmacological Research. Slavin J. Fiber and prebiotics: Mechanisms and health benefits. Nutrients [Internet]. The combination of fructooligosaccharides and resistant starch shows prebiotic additive effects in rats. Clinical Nutrition [Internet].

Savignac HM, Corona G, Mills H, Chen L, Spencer JPE, Tzortzis G, et al. Neurochemistry International [Internet]. Pyra KA, Saha DC, Reimer RA. Journal of Nutrition. Freitas KDC, Amancio OMS, Morais MB. British Journal of Nutrition [Internet].

Gobinath D, Madhu AN, Prashant G, Srinivasan K, Prapulla SG. British Journal of Nutrition. Macfarlane GT, Gibson GR, Cummings JH. Comparison of fermentation reactions in different regions of the human colon. Journal of Applied Bacteriology. Moter A, Göbel UB. Fluorescence in situ hybridization FISH for direct visualization of microorganisms.

Journal of Microbiological Methods. Olson DW, Aryana KJ. Effect of prebiotics on Lactobacillus acidophilus growth and resulting pH changes in skim milk and a model peptone system. Teixeira LS, Martim SR, Silva LSC, Kinupp VF, Teixeira MFS, Porto ALF.

Efficiency of Amazonian tubers flours in modulating gut microbiota of male rats. Tuohy KM, Probert HM, Smejkal CW, Gibson GR. Using probiotics and prebiotics to improve gut health. Drug Discovery Today. Gómez B, Gullón B, Remoroza C, Schols HA, Parajó JC, Alonso JL. Purification, characterization, and prebiotic properties of pectic oligosaccharides from orange peel wastes.

Duarte FND, Rodrigues JB, da Costa Lima M, Lima MD, Pacheco MT, Pintado MM, de Souza Aquino J, de Souza EL. Potential prebiotic properties of cashew apple Anacardium occidentale L.

Journal of Organizational Behavior. Thuaytong W, Anprung P. Food Science and Technology International. Sousa S, Pinto J, Pereira C, Malcata FX, Pacheco MTB, Gomes AM, et al.

In vitro evaluation of yacon Smallanthus sonchifolius tuber flour prebiotic potential. Studies suggest that Bifidobacteria may aid digestion, modulate the immune system and help restore microbial integrity of the gut microbiota after antibiotic treatment For those of us who have been to a beach, this may come as a surprise but those weird clumps of sea plants washed up on shore are actually edible, and believe it or not, they are extremely nutrient-dense.

Seaweed is a classification for nutrient-dense marine algae and is becoming more popular in Western diets due to its wide range of vitamins, minerals, and antioxidants. Seaweed contains both soluble and insoluble dietary fiber, along with higher concentrations of vitamins A, B1, B12, C, D, and E Jerusalem artichokes often called "sunchokes" are the tuberous root vegetables from a special species of sunflower native to central North America.

Jerusalem artichokes contain multiple prebiotic compounds such as inulin and oligofructose along with minerals such as potassium. The specific prebiotics within Jerusalem artichokes support balanced gut health by stimulating healthy bacteria which may enhance absorption of important minerals like calcium and magnesium These tasty tubers taste great roasted in the oven with a bit of salt and lemon and paired with a nut pesto recipe.

Or try them out steamed, sauteed, or boiled! Legume varieties are rich in protein, prebiotic carbohydrates, and a range of micronutrients making them a great choice for gut health and overall health and wellbeing.

Incorporating more legumes containing prebiotic carbohydrates can positively alter the gut microbiome, helping regulate intestinal movement, increase mineral absorption, and reduce obesity risk by regulating blood glucose and cholesterol levels Try preparing a homemade black bean burger or a vegetarian chili to increase your intake of legumes.

Chicory is a woody, perennial plant from the daisy and sunflower family typically with bright blue flowers. The root is cultivated and is commonly baked, ground, and used as a coffee substitute or fiber additive due to its high concentration of inulin, one of the prebiotic compounds in chicory.

Along with beneficial prebiotic compounds, chicory root has other minerals such as potassium, calcium, magnesium, selenium, and zinc The fresh roots have a bitter taste but drinking chicory root coffee or using it as a functional food ingredient when baking or making protein shakes helps most adjust to the flavor.

Onions, leeks, garlic, and spring onion are all vegetables of the Allium family all of which contain prebiotic compounds such as flavonoids shown to positively influence our gut microbiota and support immune function and metabolism All of these delicious alliums are extremely versatile in the kitchen; add them to soups, salads, or stir-fries!

Nectarines, Watermelon, and Blueberries. Most fruits are nutrient-dense sources of dietary fiber, potassium. antioxidants, carotenoids and are low-calorie Studies have found that blueberries, pears, watermelons, and nectarines contain slightly higher amounts of prebiotic compounds Fruits are higher in insoluble fiber, which benefits our digestion by supporting intestinal mobility and promoting the colonization of lactobacilli and bifidobacteria This also includes dried fruits such as dried figs and prunes.

Science suggests that whole grains containing beta-glucan may help lower LDL-cholesterol and blood triglyceride levels, and encourage the growth of beneficial gut bacteria 7. You can easily incorporate more oats into your meal plan by enjoying some overnight oats for breakfast! These greens and many other dark-leafy greens house a ton of vitamins and minerals such as magnesium.

phosphorus, calcium, vitamin C, and K You can use dandelion greens in a variety of ways, my favorite is making them into a delicious pesto but you can also: smoothie, green juice, salads, stir-fry. You might have heard the rumor about chocolate is healthy, this is partially true.

S mall amounts of dark chocolate may give you a health boost but eating more than sparing amounts could lead to high calorie and sugar consumption causing unwanted weight gain and high glucose levels Pure chocolate, or cocoa, contains antioxidants that act as free radicals in the body and help fight the effects of oxidative stress Cocao also contains prebiotic fibers, and like all the other prebiotic foods, helps feed the good bacteria in our gut You may have passed this Mexican root tuber a few times in the grocery store without even noticing it.

This delicious and crunchy root vegetable has a light brown exterior and pale white interior with a texture similar to enjoying an apple. Jicama is a great low-carb option and high in prebiotic inulin. You can roast it, saute it, or find it sliced thinly to use as a taco shell!

Enter your email address to see your results. Keep up this great momentum and crush all your nutrition goals with our app. Download the Trifecta App! A diet rich in whole foods and plenty of fruits and vegetables is bound to be rich in prebiotics which may benefit your gut and digestive health in the long run.

Water plays multiple roles in the body including supporting digestion, nutrient absorption, and your ability to burn fat for energy In fact, without water, your body cannot properly metabolize fat and carbohydrates.

Being even mildly dehydrated can negatively impact gut function, brain function, mood, heart health, and energy levels And while you can get water from food and other beverages, there is no better source than water itself.

Learn how much water you need to drink each day with this simple water intake calculator. Starting a meal prep plan is beneficial to help you track, plan, and eat a constant diet. There is no better way to increase the number of prebiotic fruits and vegetables in your diet than to start meal prepping your food.

When done correctly, meal prep can completely transform your health, even if you only choose to meal prep one meal a day or a few days a week. Trying to increase your intake of vegetables and get variety can be a challenge.

Let us do all the cooking, cleaning, and meal prepping for you while. Pick a meal plan that meets your food preferences, or save money. Omnivore vs. Vegan: Which Diet is Better? Weekly Menu Plans Values Testimonials Resources FAQ My Account Get Started. Back Meal Plans Meal Prep Classics Gift Cards.

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Mayo Clinic offers appointments in Arizona, Joint health inflammation and Minnesota and at Thermogenic exercise to burn calories Clinic Health System locations. Propertiies are foods prpoerties supplements Prebiotuc contain live Thermogenic exercise to burn calories intended to maintain or improve the "good" bacteria normal microflora in the body. Prebiotics are foods typically high-fiber foods that act as food for human microflora. Prebiotics are used with the intention of improving the balance of these microorganisms. Probiotics are in foods such as yogurt and sauerkraut. Prebiotics propertties in foods such as whole grains, bananas, greens, onions, garlic, soybeans and artichokes. Open Prebiotci peer-reviewed chapter. Submitted: 08 November Reviewed: Prebiiotic April Published: Thermogenic exercise to burn calories August Onion-based facial masks Prebiotic properties properteis. Several Prebuotic vitro and in prlperties studies have Thermogenic exercise to burn calories developed to elucidate Prebiotic properties prebiotic effect of foods, and properyies in in vivo studies, the physiological dynamics of this priperties has been studied in healthy or diseased individuals. In this chapter, the main in vitro and in vivo models developed for the study of the prebiotic potential of foods will be approached, which can be used by those planning to advance in this field of research. Modern society has changed its standard of living every decade and today, health is becoming an increasingly important personal and social value. Prevention of health problems is prioritized due to the costs associated with curative medicine, especially chronic diseases, which can be prevented by a healthier lifestyle [ 1 ].